Porcine Acute Diarrhea Syndrome Coronavirus (SADS-CoV) ke porcine enteric pathogenic coronavirus e sa tsoa sibolloa e ka bakang letšollo le nang le metsi ho likolobe tse sa tsoa tsoaloa le ho baka tahlehelo e kholo ea moruo indastering ea likolobe.Hajoale, ha ho na mokhoa o nepahetseng oa serological oa ho lekola katleho ea ts'oaetso ea SADS-CoV le ente, ka hona ho na le tlhoko e potlakileng ea ho sebelisa tlhahlobo e sebetsang ea enzyme e amanang le immunosorbent (ELISA) ho etsa khaello ena.Mona, ho ile ha hahuoa protheine ea plasmid e hlahisang SADS-CoV spike (S) e kopantsoeng le IgG Fc ea motho ho hlahisa recombinant baculovirus mme e hlahisoa liseleng tsa HEK 293F.Protheine ea S-Fc e hloekisoa ka resin ea protheine ea G mme e boloka ts'ebetso e nang le anti- human Fc le anti-SADS-CoV antibodies.Joale protheine ea S-Fc e ile ea sebelisoa ho hlahisa ELISA e sa tobang (S-iELISA) le ho ntlafatsa maemo a karabelo ea S-iELISA.Ka lebaka leo, ka ho hlahloba boleng ba OD450nm ba 40 SADS-CoV sera e mpe e tiisitsoeng ke immunofluorescence assay (IFA) le Western blotting, boleng ba ho khaola bo ile ba ikemisetsa ho ba 0.3711.Coefficient of variation (CV) ea 6 SADS-CoV positive sera ka hare le lipakeng tsa S-iELISA kaofela e ne e le ka tlase ho 10%.Teko ea cross-reactivity e bontšitse hore S-iELISA ha e na cross-reactivity le sera sa kokoana-hloko ea porcine.Ho phaella moo, ho itšetlehile ka ho fumanoa ha li-serum tsa kliniki tsa 111, tekanyo ea kakaretso ea IFA le S-iELISA e ne e le 97.3%.Teko ea ho thibela kokoana-hloko e nang le litekanyetso tse 7 tse fapaneng tsa OD450nm tsa serum e bonts'itse hore boleng ba OD450nm bo fumanoeng ke S-iELISA bo ne bo amana hantle le tlhahlobo ea tšoaetso ea kokoana-hloko.Qetellong, S-iELISA e ile ea etsoa ka lisampole tsa serum ea likolobe tse 300.Li-kits tsa khoebo tsa li-enteroviruses tse ling tsa porcine li bontšitse hore litekanyetso tse ntle tsa IgG tsa SADS-CoV, TGEV, PDCoV le PEDV e ne e le 81.7%, 54%, le 65.3%, ka ho latellana., 6%, ka ho latellana.Liphetho li bontša hore S-iELISA e totobetse, e na le kutloelo-bohloko, 'me e ka ikatisetsa, 'me e ka sebelisoa ho lemoha tšoaetso ea SADS-CoV indastering ea likolobe.Sengoliloeng sena se sirelelitsoe ke litokelo tsa molao.litokelo tsohle li sirelelitsoe.
Nako ea poso: Jun-22-2021